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KMID : 1161420140170020268
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Journal of Medicinal Food
2014 Volume.17 No. 2 p.268 ~ p.277
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Immunoadjuvant Efficacy of N-Carboxymethyl Chitosan for Vaccination via Dendritic Cell Activation
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Kim Jong-Jin
Nam Joung-Pyo
Nah Jae-Woon
Jang Mi-Kyeong
Yee Sung-Tae
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Abstract
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To induce humoral- and cell-mediated immune responses in protein-based vaccinations, immunoadjuvants are needed. In this study, we investigated the immunoadjuvant effect of N-carboxymethyl chitosan (N-CMCh) on the vaccination system through dendritic cell activation. The results showed that N-CMCh nanoparticle enhanced the secretion of cytokines (interleukin [IL]-6, IL-12p70, and tumor necrosis factor-α) and antigen uptake in bone marrow-derived dendritic cells. This activated antigen-specific Th1 cell responses, including IL-2 and interferon-gamma production and proliferation. In addition, N-CMCh delayed the dissemination of ovalbumin (OVA) from an injection site in female C57BL/6 mice. As a result, in the subcutaneous vaccination, OVA-N-CMCh nanoparticles enhanced both humoral and cell-mediated vaccine responses.
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KEYWORD
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chitosan, cytokines, immunomodulation, in vitro, in vivo
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